dc.contributor.author | Kiprono, Sabella Jelimo | |
dc.date.accessioned | 2019-03-25T09:52:31Z | |
dc.date.available | 2019-03-25T09:52:31Z | |
dc.date.issued | 2017-07-31 | |
dc.identifier.uri | http://r-library.mmust.ac.ke/123456789/1277 | |
dc.description | APPLIED MICROBIAL AND CELL PHYSIOLOGY | en_US |
dc.description.abstract | The current study reports the modification of prokaryotic
microorganism through a single-layer technique by
using different polyanions/cations and doping with magnetic
(Fe3O4) nanoparticles. Briefly, individual Escherichia coli
cells were encapsulated through deposition of 1% sodium
alginate as first layer followed by depositing precipitate layers
of calcium chloride, disodium hydrogen phosphate, and
Fe3O4 nanoparticles. Surface and cross sectional analysis of
modified E. coli cells by field emission scanning electron
microscope (FE-SEM) confirmed the synthesis of varying
sizes of artificial shells around the microbial cells while the
deposition of Fe3O4 nanoparticles was confirmed by transmission
electron microscope (TEM). Thermogravimetric analysis
(TGA) showed the deposition of 58 wt% of Fe3O4 nanoparticles
on E. coli cell surface. Chemical structure analysis by
Fourier transform infrared (FTIR) spectroscopy confirmed
the presence of characteristic functional groups of deposited
reagents in the hydrogel capsule. Zeta potential analysis of
hydrogel capsule showed moderate stability with a surface
charge of − 21 mV. Growth and viability analysis by Alamar
Blue assay indicated marked increase in the reduction of
resazurin blue (> 100%) by the modified E. coli indicating
their viability. The movement and control of magnetized
E. coli cells were manipulated using external permanent magnetic
field as observed with optical microscope images. The
surface-modified cells can find potential applications in bioremediation,
biodegradation, and catalysis and can be used as
biosorbents. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.subject | Biomimetic modification . Microorganisms . Encapsulation . Nanoparticles . Cell viability | en_US |
dc.title | Encapsulation of E. coli in biomimetic and Fe3O4-doped hydrogel: | en_US |
dc.title.alternative | structural and viability analyses | en_US |
dc.type | Other | en_US |