• Login
    View Item 
    •   DSpace Home
    • University Journals/ Articles
    • Journal Articles
    • View Item
    •   DSpace Home
    • University Journals/ Articles
    • Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Morphological Assessment and Effectiveness of Indigenous Rhizobia Isolates that Nodulate P. vulgaris in Water Hyacinth Compost Testing Field in Lake Victoria Basin

    Thumbnail
    View/Open
    Muthini452013BJAST5757.pdf (1013.Kb)
    Date
    2013-12-05
    Author
    Muthini, Morris
    Maingi, John M.
    Muoma, John O.
    Amoding, Alice
    Mukaminega, Dative
    Osoro, Newton
    Mgutu, Allan
    Ombori, Omwoyo
    Metadata
    Show full item record
    Abstract
    Aims: The study was aimed at isolating, identifying and assessing the effectiveness of indigenous rhizobia nodulating P. vulgaris in Lake Victoria Basin (LVB). Study Design: Randomized complete block design. Place and Duration of Study: Soil and nodule samples were collected from Kisumu (Kenya); Kabanyolo (Uganda) and Nyabarongo (Rwanda). Field experiments: Kisumu (Kenya). Lab and greenhouse experiments: Department of Plant and Microbial Sciences Kenyatta University (Kenya) and Makerere University (Uganda). Research was carried out between January 2012 and April 2013. Methodology: Rhizobia were isolated from nodules obtained from P. vulgaris (rose coco variety) plants planted in the LVB water hyacinth compost trial fields and whole soil trapping experiments in the greenhouse using soil obtained from the LVB. The isolates were characterized using morphological features. Isolates from each group were used in authentication using the infection technique. Results: One hundred and twenty eight isolates were obtained from the trapping experiments and placed into nine groups based on their morphological characteristics. Four hundred and seventy two isolates were obtained from the nodules of the P. vulgaris grown in soils amended with water hyacinth compost and were placed into sixteen groups. The isolates varied in their morphological characteristics. There was a significant difference in the infectiveness and effectiveness of the representative rhizobia isolates. Conclusion: The studies revealed that rhizobia isolates from Lake Victoria are different morphologically. Authentication experiments, confirmed that the majority of the isolates were rhizobia due to their ability to infect the host plant P. vulgaris. All representative isolates varied in their ability to infect and fix nitrogen. Isolates that are more effective compared to the commercial Rhizobium leguminosarum biovar phaseolistrain 446 were isolated in this study. The effective indigenous rhizobia have therefore the potential of being sources of inocula for P. vulgaris.
    URI
    https://doi.org/10.9734/BJAST/2014/5757
    https://journalcjast.com/index.php/CJAST/article/view/814
    http://ir-library.mmust.ac.ke:8080/xmlui/handle/123456789/2322
    Collections
    • Journal Articles [411]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV