Protective effect of Spirulina platensis extract on aflatoxin B1 immunotoxicities in mice
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Date
2023-02-28Author
Masese, Johnson
Kipkoech, Gilbert
Kimoloi, Sammy
Mwitari, Peter
Nguka, Gordon
Mutai, Charles
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Show full item recordAbstract
Aspergillus flavus and Aspergillus parasiticus are the main Aspergillums species that form aflatoxins.
Aflatoxins are hepatotoxic, teratogenic, carcinogenic and immunosuppressive. This study aimed to
assess Spirulina platensis (S. platensis) extract inhibitory effect against aflatoxin B1 (AFB1) induced
immunotoxicity in male Swiss albino mice. Twenty-five inbred weaned mice were randomly divided
into five groups. Group I (Control group), were given routine diet. Treatments administered were: Group
II (S. platensis extract 100 mg/kg/day), Group III (AFB1 200 µg/kg/day), Group IV (S. platensis extract
100 mg/kg/day and AFB1 200 µg/kg/day) and Group V (S. platensis extract 200 mg/kg/day and AFB1
200 µg/kg/day) for 28 consecutive days. Blood was aseptically collected and centrifuged to obtain
serum. Quantitative determination of IgG, IgM and IgA in blood serum was done using ELIZA kits.
One-way ANOVA data analysis was done. Post-hoc analysis was done using Tukey’s Honestly
Significantly Differenced (HSD). P<0.05 statistical significance level was considered significant.
Compared to group I (control), treatment with AFB1 200 µg/Kg/day (group III) led to reduced IgA
(0.7147±0.001 vs. 0.7075±0.010); reduced IgM (0.0916±0.003 vs. 0.0866±0.019) and elevated IgG
(0.1746±0.001 vs. 0.2808±0.243) mean levels. Supplementation of S. platensis extract 200 mg/Kg/day
(group V) reversed the AFB1 (200 µg/kg/day)-induced depression of IgA levels (0.7124±0.005 vs.
0.7075±0.010; P=0.05437); IgM (0.1005±0.004 vs. 0.0866±0.019; P=0.0178); as well as the induced
elevation of IgG levels (0.1749±0.001 vs. 0.2808±0.243; P=0.0155). In conclusion, immune changes in
IgG and IgM caused by AFB1 could be reversed by supplementation of S. platensis extract
URI
https://doi.org/10.31254/phyto.2023.12104https://phytopharmajournal.com/assets/pdf_files/Vol12_Issue1_04.pdf
http://ir-library.mmust.ac.ke:8080/xmlui/handle/123456789/2195
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