• Login
    View Item 
    •   MMUST Institutional Repository
    • University Journals/ Articles
    • Gold Collection
    • View Item
    •   MMUST Institutional Repository
    • University Journals/ Articles
    • Gold Collection
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Entire Expressed Peripheral Blood Transcriptome in Pediatric Severe Malarial Anemia

    Thumbnail
    View/Open
    Entire Expressed Peripheral Blood Transcriptome in Pediatric Severe Malarial Anemia.pdf (1.828Mb)
    Date
    2023-06-19
    Author
    Anyona, Samuel
    Cheng, Qiuying
    Guo, Yan
    Raballah, Evans
    Hurwitz, Ivy
    Onyango, Clinton
    Seidenberg, Philip
    Schneider, Kristan
    Lambert, Christophe
    McMahon, Benjamin
    Ouma, Collins
    Perkins, Douglas
    Metadata
    Show full item record
    Abstract
    This study on severe malarial anemia (SMA: Hb < 6.0 g/dL), a leading global cause of childhood morbidity and mortality, analyzed the entire expressed transcriptome in whole blood from children with non-SMA (Hb ≥ 6.0 g/dL, n = 41) and SMA (n = 25). Analyses revealed 3,420 up-regulated and 3,442 down-regulated transcripts, signifying impairments in host inflammasome activation, cell death, innate immune responses, and cellular stress responses in SMA. Immune cell profiling showed a decreased antigenic and immune priming response in children with SMA, favoring polarization toward cellular proliferation and repair. Enrichment analysis further identified altered neutrophil and autophagy-related processes, consistent with neutrophil degranulation and altered ubiquitination and proteasome degradation. Pathway analyses highlighted SMA-related alterations in cellular homeostasis, signaling, response to environmental cues, and cellular and immune stress responses. Validation with a qRT-PCR array showed strong concordance with the sequencing data. These findings identify key molecular themes in SMA pathogenesis, providing potential targets for new malaria therapies.
    URI
    https://doi.org/10.21203/rs.3.rs-3150748/v1
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371159/
    http://ir-library.mmust.ac.ke:8080/xmlui/handle/123456789/2415
    Collections
    • Gold Collection [969]

    MMUST Library copyright © 2011-2022  MMUST Open Access Policy
    Contact Us | Send Feedback
     

     

    Browse

    All of Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    MMUST Library copyright © 2011-2022  MMUST Open Access Policy
    Contact Us | Send Feedback